Elevation of adenosine 3′,5′-cyclic monophosphate in established mammalian cell strains by hypaque (sodium diatrizoate)
- 1 November 1975
- journal article
- research article
- Published by Springer Nature in In Vitro Cellular & Developmental Biology - Plant
- Vol. 11 (6) , 404-408
- https://doi.org/10.1007/bf02616378
Abstract
Hypaque (sodium diatrizoate), when added to cells growing in culture (two HeLa S3 strains, rat glioblastoma C6 and mouse lymphoma L5178Y), increased the intracellular level of adenosine 3′,5′-cyclic monophosphate. A transient elevation of adenosine 3′,5′-cyclic monophosphate was also observed when L5178Y cells were subjected to a procedure recommended for separation of lymphocytes from peripheral blood. The effect of Hypaque did not appear to be related to the increase in osmolality of the medium.Keywords
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