Optimization of A Reverse Phase Ion-Pair Chromatographic Separation for Drugs of Forensic Interest Part I - Variables Effecting Capacity Factors

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Abstract
A study was presented employing .apprx. 50 drugs of forensic interest to determine the effect of stationary phase, water-methanol ratio, alkyl length and concentration of counter-ion and basicity of the compound chromatographed on capacity factors utilizing a reverse phase ion-pair separation. Microbondapak-C18, Microbondapak-alkyl phenyl and Microbondapak-CN were the columns examined. The mobile phases used contained water, methanol, acetic acid and an alkylsulfonate salt. Horvath''s solvophobic theory was a useful model for explaining many of the chromatographic trends.