ELECTROPHORETIC PROFILES OF AQUEOUS‐SOLUBLE PROTEINS OF HUMAN CEREBRAL CORTEX: POPULATION AND DEVELOPMENTAL CHARACTERISTICS1
- 1 April 1974
- journal article
- Published by Wiley in Journal of Neurochemistry
- Vol. 22 (4) , 517-520
- https://doi.org/10.1111/j.1471-4159.1974.tb06887.x
Abstract
—Aqueous‐soluble brain proteins from 100 adult humans have been screened by discontinuous polyacrylamide and by continuous‐SDS‐polyacrylamide electrophoresis for evidence of genetically determined variation. In addition 42 adult Macaca nemestrina and 36 inbred strains of mice were screened in the SDS‐continuous system. No variation in position of major bands was found within species, although differences in relative intensity of staining were noted. Fetal human cerebral cortex gave profiles of aqueous‐soluble proteins distinguishable from that of adults. After affinity chromatography on Concanavalin‐A‐agarose, adult samples gave one band on SDS‐polyacrylamide gels, while fetal samples gave the same band plus an additional band, presumably representing glycoproteins.Keywords
This publication has 22 references indexed in Scilit:
- STUDIES BY DNA‐RNA HYBRIDIZATION OF TRANSCRIPTIONAL DIVERSITY IN HUMAN BRAIN1Journal of Neurochemistry, 1973
- A Limit on the Redshift due to Interaction with Electromagnetic RadiationNature, 1973
- ISOLATION AND CHARACTERIZATION OF A SOLUBLE GLUCOSE‐CONTAINING SIALOGLYCOPROTEIN FROM THE CORTICAL GREY MATTER OF CALF BRAINJournal of Neurochemistry, 1972
- BRAIN PROTEINS: QUALITATIVE AND QUANTITATIVE CHANGES, SYNTHESIS AND DEGRADATION DURING FETAL DEVELOPMENT OF THE RABBITJournal of Neurochemistry, 1972
- Average heterozygosity per locus in man: an estimate based on the incidence of enzyme polymorphismsAnnals of Human Genetics, 1972
- Demonstration of a glycoprotein which is associated with a purified myelin fraction from rat brainBiochemical and Biophysical Research Communications, 1972
- Differences in protein patterns on polyacrylamide‐gel electrophoresis of neuronal membranes from mice of different strainsJournal of Neurochemistry, 1972
- DISC ELECTROPHORETIC SEPARATION OF PROTEINS IN NEURONAL, GLIAL AND SUBCELLULAR FRACTIONS FROM CEREBRAL CORTEXJournal of Neurochemistry, 1971
- THE DISC ELECTROPHORETIC SEPARATION OF PROTEINS FROM VARIOUS PARTS OF THE GUINEA PIG BRAIN1Journal of Neurochemistry, 1970
- DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS*Annals of the New York Academy of Sciences, 1964