Structural and functional significance of association of a low molecular weight chalone from JB‐1 ascites tumours with high molecular weight factors
- 1 July 1982
- journal article
- research article
- Published by Wiley in Cell Proliferation
- Vol. 15 (4) , 457-468
- https://doi.org/10.1111/j.1365-2184.1982.tb01063.x
Abstract
The cell‐specific inhibitory (chalone) activity of JB‐1 ascites tumour cell proliferation has been purified using five different procedures. By combining (1) molecular weight estimations based on ultrafiltration and gel chromatography, and (2) partitioning in organic solvent and ion exchangers, it is concluded that the active factor associates, in a complex manner, with various other components involving both hydrophobic and ionic forces. The active factor appears to be a slightly acidic, hydrophobic peptide (molecular weight 500–1000 D). When assessing the activity in vivo, it appears to be highly dependent on associated serum factors. Thus, the chalone studied appears to interact both structurally and functionally with various associated factors which affect its physicochemical behaviour and biological activity.This publication has 19 references indexed in Scilit:
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