Isolation and characterization of a mutant from Saccharomyces cerevisiae lacking fructose 1,6‐bisphosphatase
- 3 March 1984
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 139 (3) , 651-655
- https://doi.org/10.1111/j.1432-1033.1984.tb08053.x
Abstract
Mutants lacking fructose 1,6-bisphosphatase activity were isolated from S. cerevisiae and genetically purified. Mutants were unable to grow on gluconeogenic C sources. Revertants that grew on glycerol have regained the fructose 1,6-bisphosphatase activity, showing that there is no bypass in yeast for this enzymatic step. No significant differences were found in the growth of the mutants and the parental strains in other C sources. Other mutants lacking fructose 1,6-bisphosphatase activity but pleiotropically affected in the derepression of several enzymes sensitive to catabolite repression were also isolated. All the mutants isolated were of nuclear origin and defined 3 complementation groups.This publication has 19 references indexed in Scilit:
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