Infectious stunting and leg weakness em broilers ii. Studies on alkaline phosphatase isoenzymes in blood plasma1

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Abstract
Total alkaline phosphatase (ALP) activity in blood plasma of broilers suffering from infectious stunting after artificial infection was constantly higher than that of uninoculated control birds. While total ALP activity in blood plasma from inoculated broilers of various ages decreased after incubation at 56°C, it increased in similarly treated plasma collected from broilers at 3, 21 and 28 days after inoculation. Investigations into the organ origin of the plasma ALP isoenzymes with agarose electrophoresis, column chromatography, L phenylanine inhibition and heat treatment showed that the main part of ALP activity in blood plasma of both, inoculated and uninoculated birds was most likely of intestinal origin. In 3‐day‐old broilers no heat sensitive part of total ALP could be measured.