The Use of Evan's Blue Stain to Test the Survival of Plant Cells after Exposure to High Salt and High Osmotic Pressure

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Abstract
The survival of plant cells may be tested rapidly and conveniently by staining tissue sections in a solution of Evan's blue (0·5% w/v) after exposure to solutions of high salt concentration or low osmotic potential. Living cells retain the ability to exclude Evan's blue at the plasma membrane and remain their natural colour. Cells damaged by salt or osmotic stress are unable to exclude Evan's blue, are stained deep blue, and are readily distinguished upon microscopic examination.