Microbiological Assay of Tetracycline with a Potentiometric CO2Gas Sensor
- 1 January 1982
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 15 (16) , 1345-1359
- https://doi.org/10.1080/00032718208069538
Abstract
A novel potentiometric method for the determination of the antibiotic tetracycline in pharmaceutical preparations is reported. The method is based on the inhibition of respiration of a suspension of Escherichia cole, which is measured with a potentiometric carbon dioxide sensor. The dose-response curve of carbon dioxide produced versus the logarithm of the tetracycline hydro-chloride concentration is linear from 33 to 167 μg/ml. Good agreement with the label claim was obtained in the assay of a pharmaceutical preparation of tetracycline hydrochloride.Keywords
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