Binding of different monosaccharides by lectin PA‐IIL from Pseudomonas aeruginosa: Thermodynamics data correlated with X‐ray structures
- 19 January 2006
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 580 (3) , 982-987
- https://doi.org/10.1016/j.febslet.2006.01.030
Abstract
The lectin from Pseudomonas aeruginosa (PA‐IIL) is involved in host recognition and biofilm formation. Lectin not only displays an unusually high affinity for fucose but also binds to l‐fucose, l‐galactose and d‐arabinose that differ only by the group at position 5 of the sugar ring. Isothermal calorimetry experiments provided precise determination of affinity for the three methyl‐glycosides and revealed a large enthalpy contribution. The crystal structures of the complexes of PA‐IIL with l‐galactose and Met‐β‐d‐arabinoside have been determined and compared with the PA‐IIL/fucose complex described previously. A combination of the structures and thermodynamics provided clues for the role of the hydrophobic group in affinity.Keywords
This publication has 23 references indexed in Scilit:
- Structural basis of high-affinity glycan recognition by bacterial and fungal lectinsCurrent Opinion in Structural Biology, 2005
- Structural basis for the interaction between human milk oligosaccharides and the bacterial lectin PA-IIL of Pseudomonas aeruginosaBiochemical Journal, 2005
- Pseudomonas aeruginosa lectin LecB is located in the outer membrane and is involved in biofilm formationMicrobiology, 2005
- High affinity fucose binding of Pseudomonas aeruginosa lectin PA‐IIL: 1.0 Å resolution crystal structure of the complex combined with thermodynamics and computational chemistry approachesProteins-Structure Function and Bioinformatics, 2004
- Altered terminal glycosylation and the pathophysiology of CF lung diseaseJournal of Cystic Fibrosis, 2004
- Structural Basis of Carbohydrate Recognition by the Lectin LecB from Pseudomonas aeruginosaJournal of Molecular Biology, 2003
- The Glycosylation of Airway Mucins in Cystic Fibrosis and its Relationship with Lung Infection by Pseudomonas aeruginosaPublished by Springer Nature ,2003
- Structural basis for oligosaccharide-mediated adhesion of Pseudomonas aeruginosa in the lungs of cystic fibrosis patientsNature Structural & Molecular Biology, 2002
- Specificity of the fucose-binding lectin ofPseudomonas aeruginosaFEMS Microbiology Letters, 1987
- [32] Pseudomonas aeruginosa lectinsPublished by Elsevier ,1982