Molecular cloning of two receptors from rat brain with high affinity for salmon calcitonin
- 5 July 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 325 (3) , 225-232
- https://doi.org/10.1016/0014-5793(93)81078-e
Abstract
Two receptors with high affinity for salmon calcitonin were cloned from the nucleus accumbens region of rat brain. The deduced 479 amino acid sequence of cDNA clone L2175‐D20 (designated Cla receptor) is 78% and 66% identical with those reported for human and porcine calcitonin receptors, respectively. Clone U3237‐A2 codes for a receptor (designated Clb) that is identical to Cla except for a 37 amino acid insert in the second extracellular domain. COS‐7 cells transfected with either transcript bound [125I]salmon calcitonin with high affinity (K d = 8 pM for Cla; K d = 48 pM for Clb) and responded to salmon calcitonin with increases in cAMP. Tissue distribution studies revealed Cla transcript in rat brain, skeletal muscle, kidney and lung, whereas Clb was predominantly found in brain.Keywords
This publication has 38 references indexed in Scilit:
- Biologically active salmon calcitonin-like peptide is present in rat brainBrain Research, 1992
- The structure of neuropeptide receptorsEuropean Journal of Pharmacology: Molecular Pharmacology, 1992
- Presence of calcitonin‐like immunoreactivity (iCT) in human prostate gland: Evidence for iCT secretion by cultured prostate cellsThe Prostate, 1992
- Two forms of the rat D2 dopamine receptor as revealed by the polymerase chain reactionFEBS Letters, 1990
- A second molecular form of D2 dopamine receptor in rat and bovine caudate nucleusNature, 1990
- Neuronal membrane sensitivity to a salmon calcitonin analogue with negligible ability to lower serum calciumNeuroscience Letters, 1988
- Inhibitory effects of calcitonin on adenylate cyclase activity in different rat brain areasLife Sciences, 1986
- A simple method for displaying the hydropathic character of a proteinJournal of Molecular Biology, 1982
- Calcitonin inhibition of physiological and stimulated prolactin secretion in ratsLife Sciences, 1981
- Lack of opiate receptor involvement in centrally induced calcitonin analgesiaLife Sciences, 1978