Ryanodine consumes one mole of periodic acid and gives oxoryanodine C25H33O9N. Splitting off water from ryanodine gives anhydroryanodine C25H33O8N. The analyses of these two derivatives are therefore in agreement with the formula for ryanodine C25H35O9N. Alkaline hydrolysis of ryanodine gives pyrrole-α-carboxylic acid and an alcohol C20H32O8. This alcohol splits off water to give a compound C20H30O7. Alkaline hydrolysis of oxoryanodine consumes three moles of alkali and gives a mixture of low molecular acidic products. Alkaline fusion of oxoryanodine gives a neutral aromatic nitrogen-free crystalline substance.