A simple method for the perifusion of isolated liver cells

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Abstract
The perfusion technique described in this paper offers a new and simple method for studying liver cell metabolism in a more dynamic way. It has potential use in hormonal response studies, pulsechase experiments and many other types of investigations. Finally, the perfusion technique may be applicable to other isolated cell preparations as well. The method combines some advantages of the flow-through technique with the great advantages of using isolated liver cells, apparently without disturbing the viability of the cells.