SUPERCOILED CIRCULAR DNA-PROTEIN COMPLEX IN Escherichia coli : PURIFICATION AND INDUCED CONVERSION TO AN OPEN CIRCULAR DNA FORM
Open Access
- 1 April 1969
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 62 (4) , 1159-1166
- https://doi.org/10.1073/pnas.62.4.1159
Abstract
The 23S twisted circular form of ColE(1) DNA has been isolated from Escherichia coli as a tightly associated DNA-protein complex with a sedimentation coefficient of approximately 24S. Treatment of this complex with pronase, trypsin, sodium dodecyl sulfate, Sarkosyl, or heat results in a conversion to a slower sedimenting form of 17S or 18S, as determined by centrifugation in neutral sucrose gradients. These treatments do not alter the sedimentation properties of noncomplexes supercoiled ColE(1) DNA even in the presence of the ColE(1)-protein complex. Electron microscopic analyses indicate that the decrease in sedimentation rate of the ColE(1)-protein complex after treatment with these various agents is largely owing to an induced transition of ColE(1) DNA from the supercoiled to the open circular state.Keywords
This publication has 13 references indexed in Scilit:
- Characterization of multiple circular DNA forms of colicinogenic factor E1 from Proteus mirabilisBiochemistry, 1968
- Studies of the Nature of the Inhibitory Action of Inorganic Phosphate, Fluoride, and Detergents on 5′-Adenylic Acid Deaminase Activity and on the Activation by Adenosine TriphosphateJournal of Biological Chemistry, 1968
- The interaction of closed circular DNA with intercalative dyesJournal of Molecular Biology, 1968
- CONFORMATIONAL CHANGES IN ASPARTATE TRANSCARBAMYLASE .I. PROTEOLYSIS OF INTACT ENZYME1968
- Preparation and purification of ФX-RF component IBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1968
- Lysis of Escherichia coli with a neutral detergentBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1967
- Circular DNA forms of a bacterial sex factor.Proceedings of the National Academy of Sciences, 1967
- Evidence for circular DNA forms of a bacterial plasmid.Proceedings of the National Academy of Sciences, 1967
- Physical and Topological Properties of Circular DNAThe Journal of general physiology, 1966
- The twisted circular form of polyoma viral DNA.Proceedings of the National Academy of Sciences, 1965