Structure and transcription of a human gene for H1 RNA, the RNA component of human RNase P
Open Access
- 11 January 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 18 (1) , 97-103
- https://doi.org/10.1093/nar/18.1.97
Abstract
The gene coding for H1 RNA, the RNA component of human RNase P, has been isolated and characterized from a human genomic DNA library. The sequence corresponding to the mature H1 RNA is almost identical to that previously identified using H1 RNA and a cDNA clone corresponding to it. The nucleotide sequence of the genomic clone contains an array of potential transcriptional control elements, some characteristic of transcription by RNA polymerase III and some characteristic of RNA polymerase II, as is also the case for U6 and certain other small stable RNAs. The transcription in vitro of the genomic clone shows that the gene is functional and is transcribed by RNA polymerase III. Southern hybridization analysis indicates that there is very likely only one copy of the gene for H1 RNA in the human genome.This publication has 29 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Identification and characterization of an RNA molecule that copurifies with RNase P activity from HeLa cells.Genes & Development, 1989
- The Alu family developed through successive waves of fixation closely connected with primate lineage historyJournal of Molecular Evolution, 1988
- Upstream elements required for efficient transcription of a human U6 RNA gene resemble those of U1 and U2 genes even though a different polymerase is used.Genes & Development, 1988
- The in vitro transcription of the 7SK RNA gene by RNA polymerase III is dependent only on the presence of an upstream promoterCell, 1987
- A common octamer motif binding protein is involved in the transcription of U6 snRNA by RNA polymerase III and U2 snRNA by RNA polymerase IICell, 1987
- Distinct factors with Sp1 and NF-A specificities bind to adjacent functional elements of the human U2 snRNA gene enhancer.Genes & Development, 1987
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Aspects of biochemical catalysisCell, 1984
- A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3′ border of the regionCell, 1980