Biochemical Studies on Polyamine and its Analogues

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Abstract
Preincubation with spermine, of λ, T7 and P465 phages which were sensitive to oxidized spermine, resulted in a decrease of their susceptibility to the action of oxidized spermine. Phages resistant to oxidized spermine such as T4 and φX174 became susceptible to this agent after dialysis. The mechanism of phagocidal action of oxidized spermine was examined with 32P-labelled λ phage. Oxidized spermine interfered neither with the absorption of λ phage, nor with the injection of its DNA into the host cells. The injected DNA, however, did not lead to the formation of mature phage. The interaction of oxidized spermine with the DNA of phages T4 and T7 was investigated by thermal denaturation studies. DNA treated with oxidized spermine showed the same Tm as untreated DNA. However, the treated DNA was decreased in its hyperchromicity and was renatured to a great extent, even after rapid cooling. These facts areexplained by the formation of cross-links which prevents the separation of complementary DNA strands.

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