Tapetum-specific expression of theOsg6B promoter-?-glucuronidase gene in transgenic rice
- 1 March 1997
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 16 (6) , 363-367
- https://doi.org/10.1007/bf01146774
Abstract
The promoter of an anther tapetum-specific gene,Osg6B, was fused to aβ-glucuronidase (GUS) gene and introduced into rice byAgrobacterium-mediated gene transfer. Fluorometric and histochemical GUS assay showed that GUS was expressed exclusively within the tapetum of anthers from the uninucleate microspore stage (7 days before anthesis) to the tricellular pollen stage (3 days before anthesis). This is the first demonstration of an anther-specific promoter directing tapetum-specific expression in rice.Keywords
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