CONTROL OF CLONING OF NORMAL HUMAN T-LYMPHOCYTES BY TRANSFERRIN, ALBUMIN AND DIFFERENT LECTINS
- 1 January 1978
- journal article
- research article
- Vol. 33 (3) , 495-498
Abstract
Normal human T[thymus derived]-lymphocytes could be induced to form colonies with a high cloning efficiency by seeding the cells directly in agar with normal human plasma and the lectins concanavalin [Con] A, phytohemagglutinin or pokeweed mitogen. The requirement for human plasma could be substituted, to different degrees with different lectins, by adding transferrin and albumin to fetal calf serum. This provides a useful system for identifying specific deficiencies in the requirements for normal T-lymphocyte colony formation.This publication has 17 references indexed in Scilit:
- Control of normal cell differentiation and the phenotypic reversion of malignancy in myeloid leukaemiaNature, 1978
- Partial replacement of serum by selenite, transferrin, albumin and lecithin in haemopoitec cell culturesNature, 1976
- Growth of B-lymphocytes clones in semisolid culture is mitogen dependent.The Journal of Experimental Medicine, 1976
- Induction of colony formation in vitro by human lymphocytesNature, 1976
- Identification of transferrin as a lymphocyte growth promoter in human serumExperimental Cell Research, 1972
- The induction of clones of normal mast cells by a substance from conditioned mediumExperimental Cell Research, 1966
- In vitro control of the development of macrophage and granulocyte colonies.Proceedings of the National Academy of Sciences, 1966
- THE GROWTH OF MOUSE BONE MARROW CELLS IN VITROImmunology & Cell Biology, 1966
- The cloning of normal “Mast” cells in tissue cultureJournal of Cellular and Comparative Physiology, 1965
- Destruction of mouse and rat embryo cells in tissue culture by lymph node cells from unsensitized ratsJournal of Cellular and Comparative Physiology, 1965