IDENTIFICATION OF MONONUCLEAR-CELLS IN HUMAN-BLOOD .1. QUALITATIVE AND QUANTITATIVE DATA ON SURFACE-MARKERS AFTER FORMALDEHYDE FIXATION OF THE CELLS

  • 1 January 1980
    • journal article
    • research article
    • Vol. 41  (3) , 559-566
Abstract
The technical details of a fixation procedure with formaldehyde which was applied in a direct membrane immunofluorescence technique to mononuclear cells from normal human blood are described. After separation of the cells with Ficoll-Isopaque according to Boyum, they were washed and fixed with 0.04% formaldehyde in PBS [phosphate buffered saline] for 10 min and washed again. This cell suspension can be stored at 4.degree. C for at least 24 h and the slides prepared from them at -20.degree. C for at least some months. In practice, this fixation procedure appeared to be effective in the preservation of cells and showed a number of additional advantages, such as the short handling period, including the fixation procedure and the avoidance of loss of cells. True B lymphocytes, as defined by the synthesis of Ig and the incorporation of these molecules into their cell membrane, are recognized convincingly.