Characterization of a cDNA Encoding Rat Sterol Carrier Protein2
- 1 April 1990
- journal article
- research article
- Published by Mary Ann Liebert Inc in DNA and Cell Biology
- Vol. 9 (3) , 159-165
- https://doi.org/10.1089/dna.1990.9.159
Abstract
Sterol carrier protein2 (SCP2) is a 13.2-kD protein that is thought to be involved in the intracellular transport of cholesterol. Using synthetic oligonucleotides based on the protein sequence of SCP2, a clone (SP43) was isolated from a rat liver cDNA library. The DNA sequence revealed that the cDNA could encode a polypeptide of 273 amino acids (28.9 kD) or 143 amino acids (15.3 kD) in which the carboxy-terminal 123 amino acids are identical to the SCP2 protein. RNA blot hybridization revealed that a variety of rat tissues contain a homologous RNA of a size similar to SP43 (∼1.5 kb). Levels of SCP2 mRNA increased in parallel with cytochrome P450scc mRNA in the immature gonadotropin-primed rat ovary. The isolation of a cDNA clone encoding SCP2 will facilitate studies on its role in cholesterol metabolism.This publication has 28 references indexed in Scilit:
- A mass spectrometric study of the structure of Sterol Carrier Protein SCP2 from ratliverBiochemical and Biophysical Research Communications, 1988
- 8-Bromoadenosine cyclic 3',5'-phosphate rapidly increases 3-hydroxy-3-methylglutaryl coenzyme A reductase mRNA in human granulosa cells: role of cellular sterol balance in controlling the response to tropic stimulationBiochemistry, 1988
- Regulation of sterol carrier protein2 (SCP2) levels in the soluble fraction of rat Leydig cells. Kinetics and the possible role of calcium influxMolecular and Cellular Endocrinology, 1988
- Purification of nonspecific lipid transfer protein (sterol carrier protein 2) from human liver and its deficiency in livers from patients with cerebro-hepato-renal (Zellweger) syndromeBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1987
- The synthesis and esterification of cholesterol by hepatocytes and H35 hepatoma cells are independent of the level of nonspecific lipid transfer proteinBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1985
- The primary structure of the nonspecific lipid transfer protein (sterol carrier protein 2) from bovine liverBiochemical and Biophysical Research Communications, 1985
- Determination of nonspecific lipid transfer protein in rat tissues and morris hepatomas by enzyme immunoassayBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1984
- Evidence for sterol carrier protein2-like activity in hepatic, adrenal and ovarian cytosolBiochemical and Biophysical Research Communications, 1983
- Cytosolic modulators of activities of microsomal enzymes of cholesterol biosynthesis purification and characterization of a non-specific lipid-transfer proteinBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1983
- Sterol carrier protein hypothesis: Requirement for three substrate-specific soluble proteins in liver cholesterol biosynthesisLife Sciences, 1975