The Proteolytic Enzymes of Aspergillus oryzae. IV. On the Inhibition of the Enzymes by Serum.

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Abstract
The mode of action of serum inhibitors on protease I and II is described. Evidence is presented for the existence in human serum of 2 inhibitors for protease I, one acting rapidly and reversibly, the other acting more slowly and at a rate that depends on the temperature. Two immediate inhibitors of protease II are also demonstrated, 1 acting irreversibly and the other reversibly and temperature dependently. The inhibitors were separated by continuous electrophoresis. A number of normal animal sera were assayed for their content of the different inhibitors.

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