Static and Initiator Protein-Enhanced Bending of DNA at a Replication Origin
- 19 September 1986
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 233 (4770) , 1316-1318
- https://doi.org/10.1126/science.3749879
Abstract
DNA bending has been suggested to play a role in the regulation of gene expression, initiation of DNA replication, DNA packaging, and the recognition of specific DNA sequences by proteins. It has recently been demonstrated that DNA bending can be sequence-directed. Bent DNA has also been observed as a consequence of sequence-specific binding of proteins to DNA. In this report DNA of plasmid pT181 is shown to contain a bend at the replication origin. Furthermore, this bend is enhanced by the binding of the pT181 replication initiator protein, RepC, to the origin.This publication has 18 references indexed in Scilit:
- DNA bending at adenine · thymine tractsNature, 1986
- An altered DNA conformation in origin region I is a determinant for the binding of SV40 large T antigenCell, 1986
- Conformational changes in a replication origin induced by an initiator proteinCell, 1985
- On the sequence determinants and flexibility of the kinetoplast DNA fragment with abnormal gel electrophoretic mobilitiesJournal of Molecular Biology, 1985
- DNA bending induced by cruciform formationNature, 1985
- Conformational change in the DNA associated with an unusual promoter mutation in a tRNA operon of SalmonellaCell, 1984
- The locus of sequence-directed and protein-induced DNA bendingNature, 1984
- Complete nucleotide sequence of pT181, a tetracycline-resistance plasmid from Staphylococcus aureusPlasmid, 1983
- Base sequence and helix structure variation in B and A DNAJournal of Molecular Biology, 1983
- Mechanics of sequence-dependent stacking of bases in B-DNAJournal of Molecular Biology, 1982