Radioimmunoassay of Hepatitis B Core Antigen and Antibody with Autologous Reagents1

Abstract
Anti-HBc [hepatitis B core] antigen is the most sensitive indicator of current or recent hepatitis B virus infection, hence its detection is valuable in laboratory diagnosis of the infection. Dane particles isolated from the plasma of a single chronic [human] carrier and his autologous Ig[immunoglobulin]G labeled with 125I were utilized in a solid-phase sandwich radioimmunoassay for anti-HBc. The principle of serologic neutralization of core antigen and consequent inhibition of binding of the cores to the solid-phase rendered the method to be a simple, sensitive, specific and rapid means of detecting anti-HBc.