Callus formation from cell culture protoplasts of corn (Zea mays L.)
- 1 January 1979
- journal article
- research article
- Published by Springer Nature in Theoretical and Applied Genetics
- Vol. 54 (5) , 209-214
- https://doi.org/10.1007/bf00267709
Abstract
Routine procedures for the isolation of large numbers of protoplasts from an established cell culture of Zea mays and for the induction of sustained divisions leading to secondary cell cultures have been developed. The critical factors seem to be associated with neither specific enzymatic conditions for the isolation nor specific culture conditions for the protoplasts but with the ‘quality’ of the culture used for protoplast isolation.Keywords
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