Crystallographic ab initio protein structure solution below atomic resolution
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- 16 August 2009
- journal article
- research article
- Published by Springer Nature in Nature Methods
- Vol. 6 (9) , 651-653
- https://doi.org/10.1038/nmeth.1365
Abstract
Ab initio macromolecular phasing has been so far limited to small proteins diffracting at atomic resolution (beyond 1.2 angstrom) unless heavy atoms are present. We describe a general ab initio phasing method for 2 angstrom data, based on combination of localizing model fragments such as small alpha-helices with Phaser and density modification with SHELXE. We implemented this approach in the program Arcimboldo to solve a 222-amino-acid structure at 1.95 angstrom.Keywords
This publication has 16 references indexed in Scilit:
- Ab initiophasing of proteins with heavy atoms at non-atomic resolution: pushing the size limit of solvable structures up to 7890 non-H atoms in the asymmetric unitJournal of Applied Crystallography, 2008
- Phasercrystallographic softwareJournal of Applied Crystallography, 2007
- The Crystal Structure of the Carboxy-Terminal Domain of Human Translation Initiation Factor eIF5Journal of Molecular Biology, 2006
- A modifiedACORNto solve protein structures at resolutions of 1.7 Å or betterActa Crystallographica Section D-Biological Crystallography, 2005
- Phasing at resolution higher than the experimental resolutionActa Crystallographica Section D-Biological Crystallography, 2005
- Coot: model-building tools for molecular graphicsActa Crystallographica Section D-Biological Crystallography, 2004
- Refinement of Macromolecular Structures by the Maximum-Likelihood MethodActa Crystallographica Section D-Biological Crystallography, 1997
- On the Application of the Minimal Principle to Solve Unknown StructuresScience, 1993
- Structure of oxidized bacteriophage T4 glutaredoxin (thioredoxin): Refinement of native and mutant proteinsJournal of Molecular Biology, 1992
- A theory of phase determination for the four types of non-centrosymmetric space groups 1P222, 2P22, 3P12, 3P22Acta Crystallographica, 1956