Chromatin Bridges and Origin of Multinucleate Cells in a Bovine Testicular Cell Line

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Abstract
In the course of cytological observations of a cell line of bovine testicular origin since its initiation, many chromatin bridges and binucleate cells were noticed following the apparently spontaneous occurrence of chromatid fragmentation and formation of dicentric chromosomes. Subsequently, the frequency of binucleate cells and their respective nuclear sizes were recorded from permanent slides of material of passages made prior to, during, and after the incidence of chromatid fragmentation. Since the frequency of binucleate cells rose simultaneously with that of the chromatin bridges, and since the same nuclear size was involved in most cases, the frequency of chromatin bridges was taken as an index of their contribution to the production of binucleate cells following the period of chromatid fragmentation.