Single-feature polymorphism discovery in the barley transcriptome
Open Access
- 11 May 2005
- journal article
- method
- Published by Springer Nature in Genome Biology
- Vol. 6 (6) , R54
- https://doi.org/10.1186/gb-2005-6-6-r54
Abstract
A probe-level model for analysis of GeneChip gene-expression data is presented which identified more than 10,000 single-feature polymorphisms (SFP) between two barley genotypes. The method has good sensitivity, as 67% of known single-nucleotide polymorphisms (SNP) were called as SFPs. This method is applicable to all oligonucleotide microarray data, accounts for SNP effects in gene-expression data and represents an efficient and versatile approach for highly parallel marker identification in large genomes.Keywords
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