Cell-cell interactions in conjugating Escherichia coli: purification of F pili with biological activity.
- 1 March 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (3) , 1237-1241
- https://doi.org/10.1073/pnas.75.3.1237
Abstract
A mutant of the F sex factor has been isolated that produces more F pili per cell than does the wild-type F factor. F pili have been purified in milligram amounts from cells carrying either the mutant or the wild-type sex factor. The technique described yields F pili of up to 99% purity that can specifically bind to Escherichia coli cells and that bind to and reversibly inactivate male-specific bacteriophages. The F pilin subunit has a molecular weight of 10,750 and purified F pili have a buoyant density of 1,200 g/cm3.This publication has 14 references indexed in Scilit:
- Purification and characterization of F pili from Escherichia coliBiochemistry, 1977
- Characterization of Pili Determined by Drug Resistance Plasmids R711b and R778bJournal of General Microbiology, 1977
- Identification of the structural gene for F-pilinJournal of Molecular Biology, 1976
- The Major Proteins of the Escherichia coli Outer Cell‐Envelope Membrane. Heterogeneity of Protein IEuropean Journal of Biochemistry, 1976
- Retraction of F pili.1974
- Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants.1971
- The Properties of Sex pili, The Viral Nature of “Conjugal” Genetic Transfer Systems, and Some Possible Approaches to the Control of Bacterial Drug ResistanceCRC Critical Reviews in Microbiology, 1971
- BACTERIAL CONJUGATIONAnnual Review of Microbiology, 1969
- Filamentous bacterial viruses.1969
- STAMME VON E. COLI .3. BIOLOGISCHES VERHALTEN VON FD UND FR1963