Duration of Sample Storage Dramatically Alters Expression of the Human Immunodeficiency Virus Coreceptors CXCR4 and CCR5
Open Access
- 1 March 2001
- journal article
- Published by American Society for Microbiology in Clinical and Diagnostic Laboratory Immunology
- Vol. 8 (2) , 432-436
- https://doi.org/10.1128/cdli.8.2.432-436.2001
Abstract
Expression of the chemokine receptors CXCR4 and CCR5 was monitored using EDTA-anticoagulated whole blood held for different time periods prior to fluorescent-antibody staining. When left overnight CXCR4 expression on leukocytes was substantially increased, whereas CCR5 expression was reduced. The results were similar when heparin and acid-citrate-dextrose were used as anticoagulants.Keywords
This publication has 15 references indexed in Scilit:
- Thalidomide Suppresses Up?Regulation of Human Immunodeficiency Virus Coreceptors CXCR4 and CCR5 on CD4 + T Cells in HumansThe Journal of Infectious Diseases, 2000
- CD4+T Cell Surface CCR5 Density as a Determining Factor of Virus Load in Persons Infected with Human Immunodeficiency Virus Type 1The Journal of Infectious Diseases, 2000
- CCR5 and CXCR4 chemokine receptor expression and β-chemokine production during early T cell repopulation induced by highly active anti-retroviral therapyClinical and Experimental Immunology, 1999
- Phorbol Esters and SDF-1 Induce Rapid Endocytosis and Down Modulation of the Chemokine Receptor CXCR4The Journal of cell biology, 1997
- The HIV coreceptors CXCR4 and CCR5 are differentially expressed and regulated on human T lymphocytesProceedings of the National Academy of Sciences, 1997
- HIV-1 entry into CD4+ cells is mediated by the chemokine receptor CC-CKR-5Nature, 1996
- Identification of a major co-receptor for primary isolates of HIV-1Nature, 1996
- HIV-1 Entry Cofactor: Functional cDNA Cloning of a Seven-Transmembrane, G Protein-Coupled ReceptorScience, 1996
- Identification of RANTES, MIP-1α, and MIP-1β as the Major HIV-Suppressive Factors Produced by CD8 + T CellsScience, 1995