Enzymes of the potato concerned in the synthesis of starch. 2. The separation of phosphorylase
- 1 May 1952
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 51 (2) , 186-190
- https://doi.org/10.1042/bj0510186
Abstract
After fractionating potato juice with ethanol in the preparation of Q-enzyme (see preceding abstract) the supernatant is precipitated with ethanol-citrate. It was re-extracted and precipitated with ethanol-buffer several times, then extracted with zinc acetate and precipitated with ammonium sulfate. The phosphorylase activity was 9 times greater than previous prepns. and no phosphatase, amylase or Q-enzyme activity was present.Keywords
This publication has 7 references indexed in Scilit:
- Enzymes of the potato concerned in the synthesis of starch. 1. The separation and crystallization of Q-enzymeBiochemical Journal, 1952
- Preparation and Properties of Serum and Plasma Proteins. IV. A System for the Separation into Fractions of the Protein and Lipoprotein Components of Biological Tissues and Fluids1a,b,c,dJournal of the American Chemical Society, 1946
- A specific property solubility test for protein purity and its application to the preparation of pure liver esteraseBiochemical Journal, 1946
- Recherches sur l'amidon. XXVI. Sur la phosphorylase de pommes de terreHelvetica Chimica Acta, 1944
- The reversible formation of starch from glucose-1- phosphate catalysed by potato phosphorylaseProceedings of the Royal Society of London. B. Biological Sciences, 1940
- The estimation of phosphorusBiochemical Journal, 1940