K+ transport in Vibrio alginolyticus: isolation of a mutant defective in an inducible K+ transport system

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Abstract
When grown in a synthetic medium containing more than 3 mM K+, the marine bacterium Vibrio alginolyticus exhibited a K+ transport system with apparent K m and maximum velocity (V max) of 3.0 mM and 1.5 μmol min-1 (mg cell protein)-1, respectively. The growth rate of this organism in synthetic medium containing less than 0.2 mM K+ was dependent on K+ concentration and was half-saturated at about 50 μM K+. The cells grown at low concentrations of K+ induced another K+ transport system with K m and V max values of 0.3 mM and 0.6 μmol min-1 (mg cell protein)-1 respectively. The high-affinity system appeared when cells were grown at concentrations less than 2.0 mM K+ and was fully induced at 0.1 mM K+ and below. A mutant strain (FS181) unable to grow at 0.1 mM K+ was isolated and found to be defective in the inducible K+ transport system.

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