INCREASED SURVIVAL OF CBA PLURIPOTENT HEMATOPOIETIC STEM-CELLS INVITRO INDUCED BY A MARROW STROMAL FACTOR IN SL-SLD MICE

  • 1 January 1979
    • journal article
    • research article
    • Vol. 7  (6) , 324-327
Abstract
Media conditioned by marrow adherent cells from anemic Sl/Sld and W/Wv mice increased the 24 h survival of CBA CFU2 [pluripotential stem cells] in vitro compared to fresh medium to about the same extent as marrow-conditioned medium from normal Sl+/Sl+, W+/W+ and CBA mice. Sl/Sld marrow-conditioned medium also increased the percentage of CFUs in DNA synthesis to the same extent as CBA marrow-conditioned medium. Sl/Sld mice produce a marrow stromal factor that increases survival of CFUs and the percentage of CFUs in DNA synthesis in vitro. The defective hemopoietic microenvironment of s1/S1d mice is not due to a deficiency in the production of this factor.