Characterization of OralYersinia enterocoliticaInfection in Three Different Strains of Inbred Mice

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Abstract
Several studies have highlighted differences in the resistances of various mouse strains to intravenous (i.v.) infection withYersinia enterocolitica. In particular, differences in resistance and immunological response between BALB/c and C57BL/6 mouse strains have been determined. Following i.v infection, C57BL/6 mice are more resistant toY. enterocoliticathan are BALB/c mice. However, becauseY. enterocoliticais typically a food-borne pathogen, the oral route of infection more accurately reflects the natural route of infection. Therefore, it was of interest to ascertain if the differences in resistance between mouse strains observed for an i.v. infection can be recapitulated following an oral infection. C57BL/6j, BALB/cj, and 129X1/Svj mouse strains presented no differences in 50% lethal dose (LD50) following oral infection withY. enterocolitica. Subsequent analysis of cytokine levels, bacterial colonization and immune cell populations following oral infection confirmed characteristics previously described following i.v.Y. enterocoliticainfection. All tissues analyzed from each mouse strain demonstrated a polarized Th1 cytokine profile and inflammatory cell influx throughout a 7-day course of infection. This immune response was present in all tissues and increased as bacterial colonization progressed. The lack of a differing LD50phenotype and common trends in immunological response among the three mouse strains tested suggests that oral infection is a useful model for studying the host response toY. enterocoliticainfection.

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