Generation by Insulin of a Chemical Mediator That Controls Protein Phosphorylation and Dephosphorylation
- 21 December 1979
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 206 (4425) , 1408-1410
- https://doi.org/10.1126/science.228395
Abstract
Deproteinized skeletal muscle extracts free of major nucleotides from control and insulin-treated rats were fractionated and assayed for inhibition of protein phosphorylation by cyclic adenosine monophosphate (AMP)-dependent and -independent protein kinases. A differential effect of insulin on a particular fraction was observed on cyclic AMP-dependent protein kinase but not on cyclic AMP-independent protein kinases. This fraction that inhibited cyclic AMP-dependent protein kinase also stimulated glycogen synthase phosphoprotein phosphatase. It is proposed that this fraction may contain a mediator substance generateed in the presence of insulin.Keywords
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