Fat Soluble Vitamins

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Abstract
It appears as a result of our experiments that the physical qualities of a ration which may allow irritating ingredients to lodge in the eyes of an experimental animal are factors which should be given consideration in the production and possible cure of ophthalmia. As the incidence of ophthalmia frequently leads to a secondary effect on growth, the physical state of the ration may have an effect on growth as well. It is accordingly recommended that when rations low in vitamin A are to be used for assay purposes, they should be so treated by cooking, moistening, or otherwise, as to allay their dust producing properties. In addition it follows from our observations on growth and the production and cure of ophthalmia that a basal ration consisting of cooked polished rice 80, heated casein 12, sodium chloride 1, calcium carbonate 1, and yeast, high in vitamin B, 6, constitutes an excellent basal ration for determining vitamin A in concentrates such as cod liver oil and the like. This ration, furthermore, has the advantage of low cost as its salt ingredients are especially inexpensive.

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