A mutation causing constitutive synthesis of the pyruvate dehydrogenase complex in Escherichia coli is located within the pdhR gene
- 20 December 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 336 (1) , 43-47
- https://doi.org/10.1016/0014-5793(93)81605-y
Abstract
The aceE‐aceF‐lpd genes encoding the pyruvate dehydrogenase (PDH) complex of Escherichia coli are preceded by a gene encoding a putative transcriptional regulator, PdhR (formerly designated Gen A). Enzymological tests and studies with pdhR‐lacZ and aceE‐lacZ translational fusions have shown that a constitutive mutation (acec816), which increases PDH complex synthesis to the pyruvate‐induced level in the absence of inducer, is recessive to the wild‐type pdhR gene in trans. Sequence comparisons further showed that the acec816 mutation affects a single site in the pdhR gene leading to an Arg118 (CGU) → Cys (UGU) substitution in the PdhR protein. The results support the view that synthesis of the PDH complex is regulated from the pdhR promoter of a pdhR‐aceEF‐lpd operon.Keywords
This publication has 18 references indexed in Scilit:
- A new family of bacterial regulatory proteinsFEMS Microbiology Letters, 1991
- Site-directed mutagenesis of the lipoate acetyltransferase of Escherichia coliProceedings Of The Royal Society B-Biological Sciences, 1991
- Structure, Expression, and Protein Engineering of the Pyruvate Dehydrogenase Complex of Escherichia coliaAnnals of the New York Academy of Sciences, 1989
- Genetic reconstruction and functional analysis of the repeating lipoyl domains in the pyruvate dehydrogenase multienzyme complex of Escherichia coliJournal of Molecular Biology, 1985
- Transcription analysis of the sucAB, aceEF and lpd genes of Escherichia coliMolecular Genetics and Genomics, 1985
- Improved plasmid vectors for the isolation of translational lac gene fusionsGene, 1984
- On the location of a mutation causing constitutive synthesis of the pyruvate dehydrogenase complex inEscherichia coliK-12FEMS Microbiology Letters, 1979
- Biochemical Genetics of the -Keto Acid Dehydrogenase Complexes of Escherichia coli K 12: Genetic Characterization and Regulatory Properties of Deletion MutantsJournal of General Microbiology, 1978
- Biochemical Genetics of the -Keto Acid Dehydrogenase Complexes of Escherichia coli K12: Isolation and Biochemical Properties of Deletion MutantsJournal of General Microbiology, 1977
- Regulation of Pyruvate Dehydrogenase Complex Synthesis in Escherichia coli K12. Identification of the Inducing MetaboliteEuropean Journal of Biochemistry, 1970