Changes in the expression of N‐acetylglucosaminyltransferase III, IV, V associated with the differentiation of HL‐60 cells

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Abstract
By use of a new assay method based on HPLC, GlcNAc‐transferase III, IV and V activities were determined in HL‐60 cells, differentiated HL‐60 cells and normal myeloid cells. Differentiation along the monocytic lineage with 1α,25‐dihydroxyvitamin D3 resulted in increased GlcNAc‐transferase IV and decreased GlcNAc‐transferase III activity. Differentiation along the myeloid lineage with retinoic acid resulted in a decrease in GlcNAc‐transferase III activity. Although differentiated HL‐60 cells show a changed GlcNAc‐transferase pattern, they do not resemble normal myeloid cells. Macrophages and granulocytes are characterized by a very low level of GlcNAc‐transferase III activity whereas differentiated HL‐60 cells still contain this activity. This is the first demonstration of GlcNAc‐transferase IV and V activity in a human cell.

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