Studies on Larval Fascioloides magna. II. In vitro Survival of Axenic Rediae in Amino Acids and Sugars

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Abstract
Methods were developed for obtaining rediae of the trematode Fascioloides magna from the snail Lymnaea stagnalis jugularis free from contaminating microorganisms. Survival times of such rediae were studied under simple culture conditions in 2, 4, and 6 mM_ concentrations of individual amino acids and amides, sugars, and Na-acetate. Hydroxyproline and serine gave the most consistent increases in survival time over saline controls with more variable or less significant increases shown by alanine, glycine, leucine, phenylalanine, proline, threonine, and the amide asparagine. The sugars and acetate generally showed no equivalent tendency to prolong survival.

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