Interaction of Helicobacter pylori and its fatty acids with parietal cells and gastric H+/K(+)-ATPase.
Open Access
- 1 September 1994
- Vol. 35 (9) , 1176-1180
- https://doi.org/10.1136/gut.35.9.1176
Abstract
Helicobacter pylori and the fatty acids produced by this organism were compared for their acid inhibitory activity in isolated parietal cells and their interaction with gastric H+/K(+)-ATPase. H pylori (intact organisms, sonicates, methanolic extracts, and extracts from culture medium) and the fatty acids cis 9,10-methyleneoctadecanoic acid and tetradecanoic acid inhibited at fairly high concentrations histamine- and dibutyryl cyclic adenosine monophosphate stimulated acid production in isolated parietal cells, dissipated (with a slow onset) the H+/K(+)-ATPase created H+ gradient in gastric membrane vesicles, and inhibited H+/K(+)-ATPase activity in a concentration dependent manner. The inhibitory potency of H pylori and the fatty acids in relation to H+/K(+)-ATPase depended on the amount of membrane protein. Bovine serum albumin prevented enzyme inhibition and proton dissipation from gastric vesicles. The data indicate that H pylori establishes its antisecretory action in parietal cells by blocking H+/K(+)-ATPase activity and also by a detergent action at the apical parietal cell membrane. The fatty acids cis 9,10-methyleneoctadecanoic acid and tetradecanoic acid are probably the acid inhibitory factors secreted by H pylori.Keywords
This publication has 17 references indexed in Scilit:
- Interleukin-1 is cytoprotective, antisecretory, stimulates PGE2 synthesis by the stomach, and retards gastric emptyingLife Sciences, 1991
- EFFECT OF A CAMPYLOBACTER PYLORI PROTEIN ON ACID SECRETION BY PARIETAL CELLSThe Lancet, 1989
- Omeprazole, SCH 28080 and doxepin differ in their characteristics to inhibit H+/K+-ATPase driven proton accumulation by parietal cell membrane vesiclesBiochemical Pharmacology, 1988
- Unusual Cellular Fatty Acids and Distinctive Ultrastructure in a New Spiral Bacterium (Campylobacter Pyloridis) from the Human Gastric MucosaJournal of Medical Microbiology, 1985
- Effect of substituted benzimidazoles on acid secretion in isolated and enriched guinea pig parietal cells.Gut, 1983
- Inorganic phosphate assay with malachite green: An improvement and evaluationJournal of Biochemical and Biophysical Methods, 1982
- Inhibition of gastric by unsaturated long-chain fatty acidsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1982
- A study of H+ transport in gastric microsomal vesicles using fluorescent probesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1978
- Analysis of long-chain free fatty acid binding to bovine serum albumin by determination of stepwise equilibrium constantsBiochemistry, 1971
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951