Existence of only a single functional pool of adenosine triphosphate in human erythrocytes.

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Abstract
The question of whether separate membrane and soluble pools of ATP exist in erythrocytes was examined. Phosphoglycerate kinase (EC 2.7.2.3)-derived (membrane) ATP was labeled by short-term incubation with inorganic [32P]phosphate. Pyruvate kinase (EC 2.7.1.40)-derived (soluble) ATP was not labeled under these circumstances. The specific activity of the .gamma.-phosphate of soluble ATP was then evaluated by the addition of 2-deoxyglucose and measurement of the specific activity of 2-deoxyglucose-6-[32P]phosphate formed. This specific activity was essentially the same as the overall specific activity of erythrocyte ATP .gamma.-phosphate, indicating that no functional pools of phosphoglycerate kinase-derived and pyruvate kinase-derived ATP exist in erythrocytes.