Assessment of tissue viability by near-IR spectroscopy and imaging

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Abstract
Near IR diffuse reflectance spectroscopy and imaging are used to assess tissue status following reconstructive surgery. Utilizing the differential absorption of oxy- and deoxy-hemoglobin between 670-1100 nm, tissue hemoglobin oxygen saturation changes were monitored in reverse McFarlane dorsal rat skin flaps. Significant changes in these parameters were observed upon surgical elevation of the skin flap. A significant regional variation along the skin flap was also observed. The magnitude of the drop is tissue oxygen saturation, as observed immediately following surgery, correlated with the final clinical outcome of the flap tissue. These results indicate the potential of near IR spectroscopy and imaging to monitor tissue oxygenation status and assess tissue viability following reconstructive surgery.

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