Die Mikrobestimmung der Barbiturate. Ein neues mikrotechnisches Arbeitsverfahren
- 1 January 1955
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 302 (Jahresband) , 263-267
- https://doi.org/10.1515/bchm2.1955.302.1-2.263
Abstract
A method is described for the estimation of 10-70 [mu]g of barbiturates present on filter paper. It is therefore suitable for estimation of barbiturates after paper chromatography. The spot with the substance is wetted with Hg reagent, excess reagent removed by 2 rapid washings of 5 ml on the pump, and the Hg bound in the complex is then estimated by means of dithizone at 610 m[mu] Calibration curves are presented for luminal, veronal and phanodorm. It is considered that the method can be adapted for estimation of less than 1 [mu]g barbiturate.This publication has 0 references indexed in Scilit: