Role of Calcium in Tumor Necrosis Factor- Production by Activated Macrophages
- 1 December 1996
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 120 (6) , 1190-1195
- https://doi.org/10.1093/oxfordjournals.jbchem.a021540
Abstract
The role of calcium in the production of tumor necrosis factor-α (TNF-α) by the lipopolysaccharide (LPS)-stimulated macrophage cell line, J774.1, was investigated. Flow cytometric measurement of intracellular calcium concentration ( [Ca]1)using 2-(3,6-bis(acetyl-oxy)-2,7-dichloro-9H-xanthen-9-yl)benzoic acid (fluo-3)-loaded J774.1 cells revealed that LPS at more than 0.1 μg/ml increased [Ca]1 transiently in the presence or absence of serum, and that a mixture of a calcium chelator, ethyleneglycol-bis(β-aminoethyl ether) N,N,N',N' -tetraacetic acid (EGTA), and a calcium release blocker from intracellular store sites, 8-(diethyiamino)-octyl-3,4,5-trimethoxybenzoate hydrochloride (TMB-8), inhibited the [Ca]1 response induced by LPS. In concordance with this, production of TNF-α was inhibited by EGTA and/or TMB-8. These reagents also reduced the level of TNF-α mRNA significantly. These results indicate that the transient increase of [Ca]1 plays a role in LPS-induced expression of TNF-α by the macrophage cell line.Keywords
This publication has 0 references indexed in Scilit: