The Ribosomal Serine Proteinase, Cathepsin R

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Abstract
Ribosomes contain a proteolytic activity, characterized as an endopeptidase with serine in the active center. The enzyme was given the name cathepsin R, following the recommendations of Barrett et al. for naming new proteinases. The present paper contains evidence that cathepsin R in rat liver ribosomes is present in a cryptic form. Upon dissociation of ribosomes to subunits (and to minor extent also by 0.5 M KCl washes), the cryptic proteinase is released. Activation of the released cathepsin R is effected by equilibration with 2 M NaCl/0.05 M sodium acetate, pH 4.8. The MW of free cathepsin R is 25,000-30,000.

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