X-RAY-INDUCED CELL KILLING AND MUTATIONS IN CULTURED HUMAN CELL LINES (XERODERMA PIGMENTOSUM CELLS AND HELA S3 CELLS)

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Abstract
Assay systems for radiation-induced mutations to 8-azaguanine (8AG) resistance were developed using XP2OS cells which belong to complementation group A of xeroderma pigmentosum (XP) and the [human cervical carcinoma] HeLa S3 cells. These mutation assay systems to purine analog resistance using XP2OS and HeLa S3 cells may be useful for determining the mutation frequency induced by X-rays; the data obtained by these assay systems can be used for predicting the mutation frequency expected after human exposure to low doses of ionizing radiation. A plot of induced mutation frequency against log surviving fraction yielded an approximately linear relationship for 5 cell types including human diploid cells, Chinese hamster cells and mouse lymphoma cells which were previously studied. Mammalian cells generally have the same fixed probability of mutation induction relative to the extent of damage caused by ionizing radiation.
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