Diagnosis of Salmonella Bacteria: Antibodies Against Synthetic Salmonella O‐Antigen 8 for Immunofluorescence and co‐Agglutination using Sensitized Protein A‐Containing Staphylococci
- 1 September 1979
- journal article
- research article
- Published by Wiley in Acta Pathologica Microbiologica Scandinavica Section B Microbiology
- Vol. 87B (1-6) , 29-36
- https://doi.org/10.1111/j.1699-0463.1979.tb02399.x
Abstract
An antiserum against the synthetic disaccharide abequose .**GRAPHIC**. rhamnose (AR), representative of Salmonella O-antigen 8, coupled to bovine serum albumin (BSA) was used for diagnosis of Salmonella bacteria by indirect immunofluorescence (IFL) and by co-agglutination (COA) using sensitized protein A-containing staphylococci. Among the 1150 enteric bacteria tested in IFL, the antiserum correctly identified all 99 Salmonella serogroup C2 and C3 bacteria with O-antigen 8. No fluorescence was seen with 484 Salmonella bacteria belonging to other serogroups or 567 non-Salmonella enteric bacteria. The anti-AR-BSA serum was favorable as compared to a conventional Salmonella factor O8 serum as regards titer and specificity. In the COA test, all 22 Salmonella serogroup C2 and C3 strains agglutinated strongly and within seconds, whereas no agglutination could be seen when 93 Salmonella bacteria representing other serogroups were tested.Keywords
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