MONOETHYL ESTER OF DIPICOLINIC ACID FROM BACTERIAL SPORES

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Abstract
Detection, purification and isolation of crystalline monoethyl ester of dipicolinic acid from spores of Bacillus cereus var mycoides are described. Chemical characterization and identification of the compound are reported. Confirmation of its identity was established by comparison with the authentic monoethyl ester prepared by chemical synthesis. The ester content of the spores is not yet ascertainable because treatments necessary to release the compound from a bound form also hydrolyze the ester linkage. The number of ethyl groups in spores is higher than in vegetative cells, but the evidence cannot be used yet to conclude that all or a major part of the dipicolinic acid in spores exists in esterified form.