EXPERIMENTAL PRODUCTION OF INFECTIOUS BOVINE KERATOCONJUNCTIVITIS - COMPARISON OF SEROLOGICAL AND IMMUNOLOGICAL RESPONSES USING PILI FRACTIONS OF MORAXELLA-BOVIS

  • 1 January 1976
    • journal article
    • research article
    • Vol. 40  (1) , 60-66
Abstract
The effect of vaccinating cattle and mice on the development of keratoconjunctivitis was studied. Cattle were vaccinated with whole cells, disrupted cells and pili fractions of 3 strains of M. bovis. Mice were vaccinated with pili fractions of 3 strains. The resistance of all vaccinated animals was challenged with virulent cultures of M. bovis. In an attempt to correlate the response seen after vaccination and challenge with a pili fraction of M. bovis, vaccinated cattle and mice were grouped on the basis of signs of disease manifested and compared on the basis of serological responses. Serum samples were tested for antibodies by a gel diffusion precipitin test. A greater number of the sera of resistant cattle had antibodies to the homologous pili antigen than those of vaccinated nonresistant cattle. Cattle vaccinated with disrupted cells were not resistant to infectious bovine keratoconjunctivitis and their sera lacked antibodies against the pili antigens. Vaccinated mice were more resistant to challenge exposure by homologous than heterologous cultures. A greater number of the sera of resistant mice had antibodies to pili antigens than nonresistant mice.