Changes in α‐Tubulin and Actin Gene Expression During Optic Nerve Regeneration in Frog Retina

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Abstract
The optic nerve of the bullfrog was transected and the regeneration process was investigated. We previously reported that α‐tubulin mRNA in the retina increased to a maximum 1–2 h after optic nerve transection with no specific change in actin mRNA. In the present investigation, we examined the long‐term effect of optic nerve transection. Northern blot analysis revealed that α‐tubulin mRNA increased again gradually after the rapid and transient increase and actin mRNA increased to a maximum at 7 days (more than twofold compared to the control retinas). The period during which actin mRNA reaches a maximal increase almost corresponds to the time lag between the axotomy and the initiation of axonal outgrowth. The main cytoskeletons of neuronal growth cones have been shown to consist of actin containing microfilaments. Therefore, the transient increase of actin mRNA may have a relationship to the initial out growth of axons. On the other hand, the rapid and transient increase of α‐tubulin mRNA observed in our previous studies is probably one of the initial responses of retinal ganglion cells to the axotomy, and the gradual increase in α‐tubulin mRNA observed in this study can probably be interpreted as provision of the structural materials necessary for axonal elongation.