The nucleotide sequences of two tRNAAsngenes from tobacco chloroplasts

11 November 1981

journal article
research article
Published by Oxford University Press (OUP) in Nucleic Acids Research

Vol. 9 (21) , 5601-5608
https://doi.org/10.1093/nar/9.21.5601

Abstract

Recombinant plasmids which contain EcoRI fragments of tobacco chloroplast DNA carrying tRNA genes were constructed. Plasmids pTC211 and pTC293 contain the base sequences for tRNA ^Asn in their 1.4 and 1.1 Md EcoRI fragments, respectively. These two tRNA sequences are identical and are; 5′-TCCTCAGTAGCTCAGTGGTAGAGCGGTCGGCTGTTAACCGATTGGTCGTAGGTTCGAATCCTACTTGGGGAG-3′. Each tRNA ^Asn gene is located at about 0.9 kb apart from the distal end of each 5S rRNA gene and is coded for by the DNA strand opposite from that of the rRNA genes.

Keywords

NUCLEOTIDE SEQUENCE

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