VLDL Induces Adipocyte Differentiation in ApoE-Dependent Manner
- 1 August 2003
- journal article
- research article
- Published by Wolters Kluwer Health in Arteriosclerosis, Thrombosis, and Vascular Biology
- Vol. 23 (8) , 1423-1429
- https://doi.org/10.1161/01.atv.0000085040.58340.36
Abstract
Objective— To clarify the role of very low density lipoprotein (VLDL) and apolipoprotein E (apoE) in adipogenesis, we studied newly developed hyperlipidemic obese (ob/ob;apoE−/−) mice. Because hydrolysis of VLDL is believed to be the major source of adipogenic free fatty acids, a higher plasma level of VLDL in these mice should exaggerate obesity. Methods and Results— When fed a high-fat, high-cholesterol diet, ob/ob;apoE−/− mice did not show increased body weight or an increased amount of adipose tissue in spite of increased plasma VLDL levels, whereas ob/ob mice showed an increased body weight and amount of adipose tissue, suggesting that there is a novel apoE-dependent pathway for adipogenesis. In vitro experiments using bone marrow stromal cells and 3T3-L1 cells confirmed this notion. ApoE-deficient VLDL did not induce adipogenesis, whereas normal VLDL induced adipogenesis in these cells. The incubation of apoE-deficient VLDL with recombinant human apoE restored its adipogenic activity. Tetrahydrolips...Keywords
This publication has 41 references indexed in Scilit:
- Very Low Density Lipoprotein (VLDL) Receptor-deficient Mice Have Reduced Lipoprotein Lipase ActivityJournal of Biological Chemistry, 2002
- Subcellular Compartmentalization and Trafficking of the Insulin-Responsive Glucose Transporter, GLUT4Experimental Cell Research, 2001
- Cholesterol, a Cell Size-dependent Signal That Regulates Glucose Metabolism and Gene Expression in AdipocytesJournal of Biological Chemistry, 2001
- Transcytosis of Lipoprotein Lipase across Cultured Endothelial Cells Requires Both Heparan Sulfate Proteoglycans and the Very Low Density Lipoprotein ReceptorJournal of Biological Chemistry, 2001
- Repression of TNF-α-Induced E-Selectin Expression by PPAR Activators: Involvement of Transcriptional Repressor LRF-1/ATF3Biochemical and Biophysical Research Communications, 2000
- Structure-Function Relationship of Lipoprotein Lipase-mediated Enhancement of Very Low Density Lipoprotein Binding and Catabolism by the Low Density Lipoprotein ReceptorPublished by Elsevier ,1996
- Mouse Very‐Low‐Density‐Lipoprotein Receptor (VLDLR) cDNA Cloning, Tissue‐specific Expression and Evolutionary Relationship with the Low‐density‐lipoprotein ReceptorEuropean Journal of Biochemistry, 1994
- Spontaneous Hypercholesterolemia and Arterial Lesions in Mice Lacking Apolipoprotein EScience, 1992
- Severe hypercholesterolemia and atherosclerosis in apolipoprotein E-deficient mice created by homologous recombination in ES cellsPublished by Elsevier ,1992
- Variation in susceptibility to atherosclerosis among inbred strains of miceAtherosclerosis, 1985